常山胡柚叶片DNA的抽提与分析

经反复实验，采用在抽提前加入一定量的抗氧化剂β－巯基乙醇，用冰冻的异丙醇来沉淀DNA，并增加氯仿／异戊醇抽提次数的所谓改良CTAB法，对常山胡柚叶片DNA抽提与分析，不仅成功提取到常山胡柚叶片DNA，而且所得的DNA浓度高、纯度好，20个试样的DNA苹均质量浓度为272.86μg/mL，平均每克鲜叶片可获DNA约82μg，纯度基本上都在1.4-1.8范围内，并经RAPD反应验证，完全能满足常规分子生物学分析的要求。     

马尾松水土保持林水文功能计量研究──Ⅰ．林冠截留与土壤贮水能力

对马尾松水土保持林的截水与持水功能进行了计量研究．结果表明：林冠的截水量与冠层枝叶生物量呈正相关；林分的截雨量和降雨量的关系可用Ｒｉｃｈａｒｄｓ函数较好地拟合；灌木的截水能力与其叶质叶形有关，林分枯落物不仅具有较强的特水能力，还能明显改善土壤结构；土壤的非毛管孔隙度受植被因素的显著影响．并提出了各植被因子的持水模型和土壤孔隙度预测模型．     

Mineral nutrition and adventitious rooting in microcuttings of Eucalyptus globulus

We characterized the adventitious rooting response of Eucalyptus globulus Labill. to various concentrations of calcium, nitrogen, phosphorus, iron, manganese, zinc, boron and copper. The parameters analyzed were percent rooting, root number, root length and mean rooting time. Root number and root length were significantly affected by mineral nutrition, whereas mean rooting time and rooting percentage seemed to be closely related to auxin availability. Root number was affected by calcium, nitrogen source and zinc, whereas root length was influenced by concentrations of phosphorus, iron and manganese, and by nitrogen source. Based on these results, we evaluated various combinations of several concentrations of these minerals in each rooting phase. Cuttings that were rooted in an optimized mineral nutrient medium and acclimatized to ex-vitro conditions for two months showed significantly higher survival after transplanting and drought stress than cuttings rooted in basal medium and treated in the same way.     

Variation in needle longevity of Pinus tabulaeformis forests at different geographic scales

Needle longevity of conifer species is known to increase with latitude, but little is known about intraspecific variation and associated factors within a location. Chinese pine (Pinus tabulaeformis Carr.) forests were investigated to identify patterns of needle longevity at seven sites with distinctive climatic conditions along a latitudinal gradient (33-38 degrees N) in Shaanxi province, northwest China. A demographic approach was used to quantify needle longevity as an index of entire foliage population adjusted to needle-age-specific mortality rates. There were significant differences in needle longevity of Chinese pine stands across sites and across sample plots within a site. Individual tree needle longevity ranged from 0.62 to 3.75 years for 276 samples across sites. Needle longevity increased with latitude (R2 = 0.40, P < 0.0001), but decreased with mean January temperature (R2 = 0.63, P < 0.0001). Foliage retention of Chinese pine stands at the regional level was generally associated with climatic variability, indicating that variation in needle longevity was primarily an environmental acclimation to low temperature in winter. Stand characteristics were closely associated with needle longevity at three sites located within the same climatic zone. Needle longevity was positively correlated with tree age (R2 = 0.48, P < 0.0001) and stand density (R2 = 0.26, P = 0.0015) at Huanglong and Huangling, respectively, whereas it was negatively associated with total tree height at Zhidan (R2 = 0.50, P < 0.0001). It is concluded that, at the stand and individual tree level, intraspecific variation in needle longevity is most likely a result of adaptation to patchy microsite environments.     

半干旱地区生态建设方略初探

以神华集团神府东胜矿区生态建设为例 ,探讨了半干旱地区生态建设的必要性、指导思想、原则、生态建设的内容及对策、措施等有关问题     

阻隔法防治草履蚧研究

通过对草履蚧[Drosicha corpulenta (kuwana)]进行胶环，毒环，绑扎塑料裙和毒绳等阻隔试验，结果表明，应用废机油加羊羊脂（5:1)进行涂环阻隔防治，效果最好，其粘性持续时间达到80－120d，利用“阻隔法”防治草履介的防治最佳时期应设在草履蚧卵开始孵化至上树前的1月上旬－2月上旬。     

1-没食子酸甘油酯的研制及其抗氧化性能的研究

在实验室条件下,以没食子酸和甘油为原料合成了1-没食子酸甘油酯(GG),做为油脂或油脂食品的抗氧化剂,保护油脂不因氧化腐败,延长贮存时间。合成途径是在日本高砂光正等人工作基础上进行了若干改进,使得率由45.7％提高到72.9％。以菜子油和豆油做底物,丙酯(PG)和2,6-二特丁基对甲酚(BHT)做对比抗氧化剂,用增重法评价其抗氧化性能,柠檬酸和酒石酸做增效剂,评价其增效作用,研究了浓度和分散度对诱导期的影响,探讨了结构因素对性能的影响。实验室结果表明:在本实验条件下,活性次序是GG>PG>BHT,随着浓度的增加,活性增大顺序是GG>PG>BHT。酒石酸对GG有增效作用,柠檬酸无效。溶解度对诱导期没有影响,说明了性能差异产生的原因。通过研究为简化合成途径,进行工业化生产提供了依据,并在此基础上为合成新型抗氧化剂找到一种可行方法。     

北京市居住区植物造景初探

居住区是人居环境最直接的空间，其环境的建设应以改善和维护小区生态平衡为宗旨，以人与自然共存为目标。通过分析北京市回龙观风雅园小区、塞纳维拉小区、亚运新新家园等6个住宅区绿化现状、景观现状、功能要求、人文环境，提出了住宅区的植物配置模式。     

朱家山自然保护区生态评价

朱家山自然保护区位于贵州省黔南州瓮安县，1998年对保护区进行了综合考察。其生态评价为：丰富的生物多样性；珍稀动植物的荟萃地；罕见的固体水库；泥沙及坡积物的潜在产出区；动植物区系的过渡区域；各演替阶段并存的森林群落；科研教学的理想场所。并对今后开发提出了建议。     

低效林人工改造技术浅议

从低效林概念、类型和特征分析入手，对低效林人工改造的基本原则和技术方法进行了论证，并介绍了几种成功的改造模式。